We developed a new pipeline based on Illumina sequencing platform and SOAPBarcode program to produce full-length COI barcodes at just 1/10 of current cost. This new method will hopefully further expand both taxonomic and geographic coverages of reference DNA barcodes, enabling large-scale molecular ecology studies. Liu, S.#, C. Yang#, C. Zhou#, and X. Zhou*. 2017. Filling reference gaps via assembling DNA barcodes using high-throughput sequencing – moving toward barcoding the world. GigaScience 6(12): 1-8. PDF Abstract: Over the past decade, biodiversity researchers have dedicated tremendous efforts in constructing DNA reference barcodes for rapid species registration and identification. Alt